HEPARIN RED

Heparin Red^® is a synthetic fluorescent probe that changes intensity when interacting with heparin. Supplied by our partners Redprobes in Germany the Heparin Red^® technology is available as two kits:
1. The Heparin Red^® Kit for quantification of heparin in human plasma
2. Heparin Red^® Ultra for quantification of heparin in crude heparin extraction.

The Heparin Red^® Kit measures “chemical” heparin in blood plasma at concentrations in µg/mL, not the effect of heparin on the clotting cascade ^[1].

Additionally, the Kit is developing into a tool for pharmacokinetic studies of non-anticoagulant heparins ^[2], a class of chemicals with enormous promise for future drug therapies.

CONVENIENT – mix-and-read fluorescence assay for microplates
SENSITIVE – quantification down to low µg/mL with detection of heparins in only 20 µL plasma
VERSATILE – detection of non-anticoagulant heparins and other sulphated polysaccharides
QUALITY – robust, protein-free reagents

[1] Warttinger U, Giese C, Harenberg J, Holmer E, Krämer R. A fluorescent probe assay (Heparin Red) for direct detection of heparins in human plasma. Anal. Bioanal. Chem. 2016, 408, 8241-8251.

[2] Galli M, Magen H, Einsele H, Chatterjee M, Grasso M, Specchia G, Barbieri P, Paoletti D, Pace S, Sanderson R, D, Rambaldi A, Nagle A. Roneparstat (SST0001), an innovative heparanase (HPSE) inhibitor for multiple myeloma (MM) therapy: first in man study. Blood 2015, 126, 3246-3246.

Heparin Red^® Ultra

Heparin Red^® Ultra is a simple and easy to use assay for measuring chemical heparin in complex matrices

The 2008 heparin adulteration crisis highlighted the need for a rapid, reliable method for screening raw heparin extractions – the Heparin Red^® Ultra kit meets this need. The kit is ideal for testing heparin concentrations in crude animal extracts, and so will be of significant value to the heparin manufacturing industry ^[1].

The assay measures “chemical” heparin concentrations in µg/mL, not the effect of heparin on the clotting cascade. Heparin Red^® Ultra was reported to suppress interference by nucleic acids and detect heparin in urine ^[1].

In the publication, we only speculate about the application for crude animal extracts but have not used such samples, we have used “model solutions” that contain heparin spikes instead.

CONVENIENT – mix-and-read fluorescence assay for microplates
SENSITIVE – small sample volumes with quantification in the low µg/mL range
QUALITY – robust, protein-free consistent reagents

[1] Warttinger U, Krämer R. Quantification of heparin in complex matrices (including urine) using a mix-and-read fluorescence assay.arXiv:1611.02482